Optical measurement of transmembrane potential in intact cardiac tissues using fluorescent resonance energy transfer

TitleOptical measurement of transmembrane potential in intact cardiac tissues using fluorescent resonance energy transfer
Publication TypeJournal Article
Year of Publication1998
AuthorsLoSicco T, Malkin RA
JournalComputers in Cardiology
Start Page573
Pagination573 - 576
Date Published01/1998
Abstract

Fluorescent Resonance Energy Transfer [FRET] is the transfer of excitation energy from a donor fluorescent molecule to an acceptor fluorescent molecule. FRET has been developed to achieve fast, ratiometric, transmembrane potential sensitive fluorescence changes in single cells. The mechanism relies on the rapid redistribution of Hydrophobic fluorescent onions. These onions redistribute from one face of the plasma membrane to the other according to the Nernst equation. A second fluorophore is used to label an exatracelluar surface and undergoes energy transfer with the membrane bound sensor. During membrane depolarization the FRET is disrupted. This large change in fluorescence allows for imaging of the transmembrane potential. It is the objective of this work to adapt the FRET measurement system for eventual use in intact cardiac tissues. Currently, studies are underway for the individual verification of the functionality of each dye placed in the FRET system. The fluorescent anion experiments have shown the expected decrease in fluorescent intensity with a decrease in transmembrane potential.

Short TitleComputers in Cardiology